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- Title
Preparing oligopeptides from broken rice protein by ultrafiltration-coupled enzymatic hydrolysis.
- Authors
Wang, Hongbin; Wang, Jing; Lv, Zhijia; Liu, Yihan; Lu, Fuping
- Abstract
The resource of broken rice is quite abundant, but its exploiting and comprehensive utilization has not been achieved effectively. Producing rice oligopeptides is thought to be a promising way because of their high nutrition value and great health function. At present, the preparation of rice peptides mainly use batch enzymatic hydrolysis. Due to its uncontrollable hydrolysis process, not only oligopeptides but also large quantities of non-oligopeptides were produced. In this study, a manner of ultrafiltration-coupled enzymatic hydrolysis, combining enzymatic hydrolysis with ultrafiltration, was attempted and compared with the batch manner. Moreover, a strategy of pre-hydrolysis was used to solve the conflict between the dissolving pH of rice protein and the optimal pH of enzymatic hydrolysis. The result demonstrated that the ultrafiltration-coupled enzymatic hydrolysis manner had great advantages in producing oligopeptides, increasing the content of oligopeptides remarkably to above 60 % from below 40 % by batch enzymatic hydrolysis, displaying excellent stability in the molecular weight distribution of harvested peptides at different hydrolysis time and obtaining more antioxidant activity than batch enzymatic hydrolysis. This study made a successful attempt and laid the foundations for further studies of applying this method in the actual production technology of rice oligopeptides.
- Subjects
OLIGOPEPTIDES; RICE proteins; ULTRAFILTRATION; HYDROLYSIS; RICE enzymes; HYDROGEN-ion concentration; MOLECULAR weights
- Publication
European Food Research & Technology, 2013, Vol 236, Issue 3, p419
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-012-1904-7