We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of a microchip electrophoresis-based, high-throughput PCR-RFLP method to type Tax 233 variants of bovine leukemia virus in Japan.
- Authors
Ochiai, Chihiro; Katagiri, Yuzuru; Kobayashi, Sota; Naitoh, Ikunori; Yoneyama, Syuji; Tomita, Keisuke; Dongze, Leng; Hikono, Hirokazu; Murakami, Kenji
- Abstract
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR–RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than 233P-Tax in cattle in Japan. The proportion infected with BLV carrying the gene encoding 233L-Tax was significantly higher in Holstein cattle than in Japanese Black cattle. Holsteins infected with BLV encoding 233L-Tax had higher proviral loads than did Holsteins infected with BLV encoding 233P-Tax and Japanese Blacks infected with BLV encoding 233L-Tax or 233P-Tax. The novel method developed in this study will be a useful tool for identifying cattle harboring BLV with a higher risk of EBL and viral transmission.
- Subjects
JAPAN; BOVINE leukemia virus; HOLSTEIN-Friesian cattle; MICROCHIP electrophoresis; INTEGRATED circuits; VIRAL transmission; CAPILLARY electrophoresis
- Publication
Archives of Virology, 2020, Vol 165, Issue 12, p2961
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-020-04842-w