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- Title
QTL mapping of maize grain quality under different pollination methods.
- Authors
LI Ranran; ZHANG Xiuying; LI Ting; YANG Bingpeng; YU Ruisu; LI Dongmei; LI Qin; XU Shutu
- Abstract
[Objective] The aim of this study was to reveal the influence of pollination methods on grain quality traits and provide basis for grain quality improvement and molecular breeding through QTL mapping of maize grain quality under different pollination methods. [Method] Excellent maize inbred lines KA105 and KB020 as well as 201 F5:6 recombinant inbred lines (RILs) constructed with them were planted in Yulin, Hanzhong and Yangling, Shaanxi in 2019. The open pollinated ears from Yulin and Hanzhong and self-pollinated ears from Hanzhong and Yangling were collected for QTL mapping of maize quality including protein, oil, starch and fiber using inclusive composite interval mapping (ICIM) method. [Result] Protein, oil, starch and fiber contents of RILs population were different between the two pollination methods. Protein and fiber contents of RILs population under open pollination were significantly lower than those under self-pollination, while oil and starch contents were significantly higher than those under self-pollination. The results of variance analysis showed that there were significant differences in quality traits among different families and environments under the two pollination methods. Correlation analysis showed that grain quality traits of RILs population under open pollination were significantly positively correlated with those under self-pollination. Protein content was significantly negatively correlated with starch content, but was significantly positively correlated with fiber content. A total of 16 QTLs were detected, of which 6 were detected under open pollination explaining 5.84%-14.56% of phenotypic variation and 10 were detected under self-pollination contributing to 5.69%-14.70% of phenotypic variation. [Conclusion] Two QTLs of protein content (qPro9-1 and qPro10) were detected simultaneously under the two pollination methods. Two QTLs of starch content (qOSta10-1 and qSSta10-2) were detected respectively at two adjacent positions on chromosome 10 under the two pollination methods. One specific QTL of fiber content was detected under open pollination and one was detected under self-pollination. Two and three QTLs of oil content were detected under open pollination and self-pollination conditions, respectively.
- Publication
Journal of Northwest A & F University - Natural Science Edition, 2021, Vol 49, Issue 11, p115
- ISSN
1671-9387
- Publication type
Article
- DOI
10.13207/j.cnki.jnwafu.2021.11.015