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- Title
Derivation of pluripotent stem cells from cultured human primordial germ cells.
- Authors
Qu, W.; Tan, J.; Jiang, L. I.
- Abstract
Introduction: To investigate the method of culturing human primordial germ cells (PGC) and differentiation in vitro and in vivo. Materials/Methods: Embryos were donated by couples undergoing drug abortion, having obtained their informed consent and the approval of the Ethics Committee. Gonadal ridges and mesenteries containing (PGC, 5-7 weeks' postfertilization) were cultured on human endometrial fibroblast feeder layers. Throughout the culture period most cells within the colonies continued, alkaline phosphatase (AKP) activity staining, stem cell surface specific marker test, karyotyping were conducted. The in-vivo experiment was divided. Athymic mouse were inoculated by the suspension of stem cell to observe the teratoma formation. Result: Cultured the PGC successfully in human endometrial fibroblast feeder layer ascends for 4 months (14 passages). PGC gave rise to large multicellular colonies resembling those of human pluripotent stem cells termed embryonic stem cells. It was demonstrated that human endometrial fibroblast can be used as EG feeding cell, Pluripotent stem cells from cultured human primodial germ cells have been continuously passaged and keep undifferent ability and pluripotent. It expressed SSEA-1 SSEA-3, TRA-1-60, TRA-1-81, and OCT-4, a normal karyotype 46,XX. and formed embryonic body. Conclusion: Human primordial germ cells may be cultured on human endometrial fibroblast feeder layers.
- Subjects
GERM cells; ALKALINE phosphatase
- Publication
Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2008, Vol 16, Issue S2, pS-33
- ISSN
1472-6483
- Publication type
Abstract
- DOI
10.1016/S1472-6483(10)61545-3