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- Title
Characterization of Xanthomonas citri pv. citri from China based on spoligotyping.
- Authors
Wenting Li; Zhenxi Li; Jiaquan Huang; Meirong Xu; Zheng Zheng; Xiaoling Deng
- Abstract
Xanthomonas citri pv. citri (Xcc), a gram-negative bacterium, is the causal agent of citrus canker, one of the most devastating diseases threatening the citrus industry worldwide. Understanding the diversity and population structure of Xcc is a prerequisite for disease epidemiological monitoring and effective disease management. Recent characterization of the clustered regularly interspaced short palindromic repeats (CRISPR)/cas (CRISPR-associated proteins genes) system with a highly variable repeat number among species provides a new molecular typing method for bacterial genetic analysis. In this study, we performed systematic in silico analyses of 28 Xcc genomes and identified a credible CRISPR/cas in Xcc strains. Further analysis of CRISPR polymorphisms (repeat number and spacer types) in 129 Xcc A strains collected from six provinces in China identified 15 types of CRISPR arrays with 25 spacers. Phylogenetic analysis of Xcc strains based on the CRISPR locus produced a more reliable and accurate typing result compared to the commonly used loci. In addition, seven associated cas genes--cas 1, cas 2, cas 3, cas 4, cas 5, cas 7 (csd 2), and cas 8 (csd 1)--were found located adjacent to the CRISPR array. BLAST results showed > 99% similarity of seven cas genes among Xcc strains. Homology analysis of spacer sequences showed that six spacers had possible phage/prophage origin. The characterization of the CRISPR/cas system among Xcc strains provided an updated strain typing method for Xcc diversity analysis and yielded a panoramic view of CRISPR evolution for further studies of Xcc -phage interactions.
- Subjects
XANTHOMONAS campestris; CRISPRS; BACTERIAL genetics; HOMOLOGY (Biology); EPIDEMIOLOGY
- Publication
Horticulture Plant Journal, 2022, Vol 8, Issue 6, p727
- ISSN
2095-9885
- Publication type
Article
- DOI
10.1016/j.hpj.2022.02.003