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- Title
Increased Na<sup>+</sup>,K<sup>+</sup>-ATPase activity in the rat brain after meningitis induction by Streptococcus pneumoniae.
- Authors
Barichello, Tatiana; Generoso, Jaqueline S.; Cipriano, Andreza L.; Casagrande, Renata; Collodel, Allan; Savi, Geovana D.; Scherer, Emilene B. S.; Kolling, Janaína; Wyse, Angela T. S.
- Abstract
Barichello T, Generoso JS, Cipriano AL, Casagrande R, Collodel A, Savi GD, Scherer EBS, Kolling J, Wyse ATS. Increase Na+,K+-ATPase activity in the rat brain after meningitis induction by Streptococcus pneumoniae. Background: Pneumococcal meningitis is the most severe infection of the central nervous system with a mortality rate up to 20% and an adverse neurological result in up to 50% of survivors. A complicated series of interactions among the host immune response and oxidants seems to be responsible for meningitis associated brain dysfunctions. Na+,K+-ATPase is an essential enzyme responsible for generating and maintaining the membrane potential necessary for neural excitability, however, the Na+,K+-ATPase activity is altered in several illness; Objective: The aim of this study is to evaluate the Na+,K+-ATPase activity in hippocampus and cortex of the rats submitted to pneumococcal meningitis. Methods: Animals received 10 µl sterile saline as a placebo or an equivalent volume of Streptococcus pneumoniae to the concentration of 5 × 109cfu/ml and were killed at 24, 48, 72 and 96 h after meningitis induction. The brain structures, hippocampus and cortex, were immediately isolated on dry ice and stored at −80°C to analyse Na+,K+-ATPase activity. Results: In the hippocampus, we verified the increase of Na+,K+-ATPase activity at 48, 72 and 96 h ( p < 0.05) and in the cortex at 24 h ( p < 0.05) after pneumococcal meningitis induction. Conclusion: The Na+,K+-ATPase activity is under the control of a diversity of intracellular messengers that are able to modulate the function of the particular isozymes in a precise way. Furthermore, we verified that pneumococcal meningitis increased the Na+,K+-ATPase activity in hippocampus and cortex; this increase can be correlated with a compensatory mechanism in illness pathophysiology.
- Subjects
MENINGITIS; STREPTOCOCCUS pneumoniae; INFECTION; CENTRAL nervous system; BRAIN diseases
- Publication
Acta Neuropsychiatrica, 2012, Vol 24, Issue 5, p301
- ISSN
0924-2708
- Publication type
Article
- DOI
10.1111/j.1601-5215.2011.00635.x