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- Title
PRELIMINARY RESEARCH CONCERNING THE OBTAINING OF HERBAL EXTRACTS WITH POTENTIAL NEUROPROTECTIVE ACTIVITY.
- Authors
GÎRD, CERASELA ELENA; DUŢU, LIGIA ELENA; COSTEA, TEODORA; NENCU, IOANA; POPESCU, MARIA LIDIA; TUDOREL, OLARU OCTAVIAN
- Abstract
The aim of our research was obtaining, characterization and evaluation of the antioxidant activity, cytotoxic properties of a selective dry extract from indigenous Origanum vulgare L. (oregano), which might be used for the formulation of a phytomedicine with neuroprotective activity. Oregano phytochemical analysis was based on the HPLC determination of rosmarinic acid (identification, quantification) and spectrophotometric assays of flavonoids, phenolcarboxylic acids and total phenols. The antioxidant capacity was determined using DPPH and ferric reducing power activity methods. The dry selective extract (obtained with 50% ethanol) contains: 4.21 g flavones (expressed as rutin)/100 g dry extract, 52.67 g phenolcarboxylic acids (expressed as chlorogenic acid)/100 g dry extract, 27.30 g polyphenols (expressed as tannic acid)/100 g dry extract and 5.76 g rosmarinic acid/100 g dry extract. It has a good antioxidant activity (EC50 = 2.62 μg/mL - DPPH method and EC50 = 104.5 μg/mL - reducing power). Cytotoxic properties against Daphnia magnia invertebrate were observed only at high concentrations, above 500 μg/mL. Our results offer encouraging premises for the formulation of a phytomedicine with potential neuroprotective activity.
- Subjects
OREGANO; NEUROPROTECTIVE agents; HERBAL medicine; ANTIOXIDANTS; POLYPHENOLS; DAPHNIA magna; THERAPEUTICS
- Publication
Farmacia, 2016, Vol 64, Issue 5, p680
- ISSN
0014-8237
- Publication type
Article