We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Assessment of Mitochondrial Biogenesis and mTORC1 Signaling During Chronic Rapamycin Feeding in Male and Female Mice.
- Authors
Drake, Joshua C.; Peelor, Frederick F.; Biela, Laurie M.; Watkins, Molly K.; Miller, Richard A.; Hamilton, Karyn L.; Miller, Benjamin F.
- Abstract
Chronic inhibition of the protein synthesis regulator mTORC1 through rapamycin extends life span in mice, with longer extension in females than in males. Whether rapamycin treatment inhibits protein synthesis or whether it does so differently between sexes has not been examined. UM-HET3 mice were fed a control or rapamycin-supplemented (Rap) diet for 12 weeks. Protein synthesis in mixed, cytosolic (cyto), and mitochondrial (mito) fractions and DNA synthesis and mTORC1 signaling were determined in skeletal muscle, heart, and liver. In both sexes, mito protein synthesis was maintained in skeletal muscle from Rap despite decreases in mixed and cyto fractions, DNA synthesis, and rpS6 phosphorylation. In the heart, no change in protein synthesis occurred despite the decreased DNA synthesis. In the heart and liver, Rap males were more sensitive to mTORC1 inhibition than Rap females. In conclusion, we show changes in protein synthesis and mTORC1 signaling that differ by sex and tissue.
- Subjects
MTOR protein; MITOCHONDRIA formation; RAPAMYCIN; PROTEIN synthesis; MUSCLE protein metabolism; PHYSIOLOGICAL aspects of aging; PHOSPHORYLATION; DNA synthesis
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2013, Vol 68, Issue 12, p1493
- ISSN
1079-5006
- Publication type
Article
- DOI
10.1093/gerona/glt047