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- Title
Transcriptome Analysis via RNA Sequencing Reveals the Molecular Mechanisms Underlying the Hedera helix Response to High Temperature.
- Authors
Ting Zhang; Ping Li; Jiali Wei
- Abstract
Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress. Two Hedera helix cultivars, heat-tolerant (HT) 'Jessica' and heat-sensitive (HS) 'Shamrock', were used for differences analyses of transcriptome. We detected 6179 differentially expressed genes (DEGs) and 5992 DEGs in 'Jessica' and 'Shamrock' to heat stress, respectively. Among these, 1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties, resulting in enhancement of various pathways such as biosynthesis of secondary metabolites, glyoxylate dicarboxylate metabolism, and protein processing in endoplasmic reticulum (PPER), RNA transport, respectively. Among the common downregulated DEGs, 72 TFs in 25 gene families were found, including members of the MYB and MYBrelated families, the bHLH family, etc. In 'Jessica' (HT), 634 unique up-regulated DEGs were identified, including genes associated with phenylpropanoid biosynthesis, starch and sucrose metabolism, biosynthesis of amino acids. Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature. Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR. This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance, which provided theoretical references for improving the heat tolerance of ivy.
- Subjects
TRANSCRIPTOMES; RNA sequencing; HIGH temperatures; ENGLISH ivy; PHYSIOLOGICAL effects of heat
- Publication
Phyton (0031-9457), 2022, Vol 91, Issue 11, p2403
- ISSN
0031-9457
- Publication type
Article
- DOI
10.32604/phyton.2022.022421