We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A New Construct for Cloning DNA and Modeling the Structure of Drosophila melanogaster Polytene Chromosomes.
- Authors
Zimin, P. I.; Gortchakov, A. A.; Demakov, S. A.; Zhimulev, I. F.
- Abstract
Modification of P-element-based transformation vector pCaSpeR3 yielded a new construct, pICon, which contains the structural region of the Escherichia coli lacZ, the adjacent 5′ and 3′ regulatory regions of hsp70, pUC19, and two tandem FRTs. Owing to the hsp70 promoter, the pICon insertion site may be located on polytene chromosomes after heat shock by light or electron microscopy. The pUC19 sequence with a polylinker allows cloning of the genomic sequence adjacent to the 3′ end of pICon by P-target rescue. Functional FRTs allow insertion or deletion of various DNA fragments. The construct is large (22,046 bp), forms easily detectable structures in polytene chromosomes, and may be used to study the structural and functional organization of the Drosophila melanogaster genome, in particular, to elucidate the causes of banding pattern formation. To map the molecular boundaries of interband 3C6/C7, the DNA sequence of this region was cloned between the two FRTs.
- Subjects
CLONING; DNA; ESCHERICHIA; DROSOPHILA melanogaster; CHROMOSOMES; GENETICS
- Publication
Molecular Biology, 2004, Vol 38, Issue 2, p205
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1023/B:MBIL.0000023736.04839.56