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- Title
Expression ratio of histone demethylase KDM3A to protamine-1 mRNA is predictive of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia.
- Authors
Javadirad, S. M.; Hojati, Z.; Ghaedi, K.; Nasr‐Esfahani, M. H.
- Abstract
To evaluate the predictive value of histone demethylase KDM3A to protamine 1 (PRM1) mRNA expression ratio as a reliable marker of sperm retrieval in men with obstructive and non-obstructive azoospermia (NOA). Fifty eight azoospermic men, including 44 with NOA and 14 with obstructive azoospermia ( OA). Testis tissue samples were collected from azoospermic men who have been referred for testicular sperm extraction (TESE) and micro- TESE. Relative expression ratio of KDM3A to PRM1 was analyzed after selection of approved reference genes. Histological classification of testis biopsies was performed. Sperm retrieval following TESE and micro- TESE was evaluated. A sperm retrieval prediction sensitivity of 95% was established when the Cq of PRM1 became smaller than the Cq of both KDM3A and GAPDH genes. However, azoospermic men with down-regulated KDM3A and decreased expression of PRM1 mRNA showed very low success for sperm retrieval (<25%), even after micro- TESE surgery. The KDM3A to PRM1 mRNA expression ratio can be used as a reliable marker of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia with 95% sensitivity.
- Subjects
HISTONE demethylases; PROTAMINES; MESSENGER RNA; SPERMATOZOA; TESTIS
- Publication
Andrology, 2016, Vol 4, Issue 3, p492
- ISSN
2047-2919
- Publication type
Article
- DOI
10.1111/andr.12164