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- Title
New Advice on an Old Topic: Buffers in Reversed-Phase HPLC.
- Authors
Lupo, Sharon; Kahler, Ty
- Abstract
Buffers are commonly used in reversed-phase liquid chromatography (LC) to control the ionization state of analytes. However, the addition of buffers is much more complex than simple pH control. Complex equilibria exist between these mobile-phase additives, the analytes, the silica surface, and even the stationary phase in certain circumstances. The addition of mass spectrometry (MS) as a primary detection technique makes decisions about mobile-phase additives even more crucial. In this column instalment, we use a model set of analytes and selected applications to demonstrate the effects that buffers can have not only on the selectivity of a separation, but also on the sensitivity of a reversed-phase analysis when using MS detection.
- Subjects
HIGH performance liquid chromatography; BUFFER solutions; CHROMATOGRAPHIC analysis; IONIZATION of gases; MASS spectrometry
- Publication
LC-GC Europe, 2017, Vol 30, Issue 7, p362
- ISSN
1471-6577
- Publication type
Article