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- Title
Blood pressure-lowering effects of a Bowman-Birk inhibitor and its derived peptides in normotensive and hypertensive rats.
- Authors
de Freitas, Maria Alzira Garcia; Amaral, Nathalia Oda; Álvares, Alice da Cunha Morales; de Oliveira, Sandriele Aires; Mehdad, Azadeh; Honda, Diego Elias; Bessa, Amanda Sá Martins; Ramada, Marcelo Henrique Soller; Naves, Lara Marques; Pontes, Carolina Nobre Ribeiro; Castro, Carlos Henrique; Pedrino, Gustavo Rodrigues; de Freitas, Sonia Maria
- Abstract
Bioactive plant peptides have received considerable interest as potential antihypertensive agents with potentially fewer side effects than antihypertensive drugs. Here, the blood pressure-lowering effects of the Bowman-Birk protease inhibitor, BTCI, and its derived peptides, PepChy and PepTry, were investigated using normotensive (Wistar-WR) and spontaneously hypertensive rats (SHR). BTCI inhibited the proteases trypsin and chymotrypsin, respectively, at 6 µM and 40 µM, a 10-fold greater inhibition than observed with PepTry (60 µM) and PepChy (400 µM). These molecules also inhibited angiotensin converting enzyme (ACE) with IC50 values of 54.6 ± 2.9; 24.7 ± 1.1; and 24.4 ± 1.1 µM, respectively, occluding its catalytic site, as indicated by molecular docking simulation, mainly for PepChy and PepTry. Gavage administration of BTCI and the peptides promoted a decrease of systolic and diastolic blood pressure and an increase of renal and aortic vascular conductance. These effects were more expressive in SHR than in WR. Additionally, BTCI, PepChy and PepTry promoted coronary vasodilation and negative inotropic effects in isolated perfused hearts. The nitric oxide synthase inhibitor blunted the BTCI and PepChy, with no cardiac effects on PepTry. The findings of this study indicate a therapeutic potential of BTCI and its related peptides in the treatment of hypertension.
- Subjects
BOWMAN-Birk inhibitor; BLOOD pressure; PEPTIDES; BIOACTIVE compounds; ANTIHYPERTENSIVE agents; MOLECULAR docking
- Publication
Scientific Reports, 2020, Vol 10, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-020-66624-3