We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of Fis-1 and miR-484 Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Cancer.
- Authors
Namvar, Leila; Nadiri, Mehdi; Mohebbi, Zahra; Asadi, Milad; Seyyedi, Maryam; Amiri-Sadeghan, Amir; Soleimani, Zahra; Hashemzadeh, Shahriyar; Khalili, Yeganeh; Zarredar, Habib
- Abstract
Background: MicroRNAs (miRNAs) regulate gene expression and various cellular activities. They also hold significant importance in the progression and development of human malignancies. Among these, miRNA-484 and the Fis-1 gene have been identified as having substantial roles in lung cancer. This study aims to ascertain miRNA-484 and Fis-1 gene expression levels in non-small cell lung cancer (NSCLC) patients. Method: In this case-control study, 45 pairs of tumor tissues and their corresponding healthy margin tissues were surgically obtained from NSCLC patients and promptly preserved in liquid nitrogen after excision. Total RNA extraction was performed using TRIzol, followed by cDNA synthesis using a designated kit. Afterward, we used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure the expression levels of miRNA-484 and the Fis-1 gene. Furthermore, the clinico-pathological characteristics of the NSCLC patients were assessed. Results: Our findings revealed an upregulation of miRNA-484 expression and downregulation of Fis-1 gene expression in NSCLC tissues compared with non-tumor tissues. Additionally, significant correlations were observed between miRNA-484 and Fis-1 gene expression levels and clinicopathological features of the patients, including factors such as lymph node involvement and distant metastasis. Conclusion: These findings suggest the potential utility of Fis-1 and miR-484 as prognostic and diagnostic markers in NSCLC.
- Subjects
LUNG cancer; PROTEINS; MICRORNA; LUNG tumors; CASE-control method; LYMPH nodes; GENE expression; NITROGEN; POLYMERASE chain reaction; TUMOR markers
- Publication
Middle East Journal of Cancer, 2024, Vol 15, Issue 1, p1
- ISSN
2008-6709
- Publication type
Article
- DOI
10.30476/mejc.2023.96826.1839