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- Title
Molecular and Biological Characterization of a New Isolate of Guinea Pig Cytomegalovirus.
- Authors
Schleiss, Mark R.; McAllister, Shane; Armién, Anibal G.; Hernandez-Alvarado, Nelmary; Fernández-Alarcón, Claudia; Zabeli, Jason C.; Ramaraj, Thiruvarangan; Crow, John A.; McVoy, Michael A.
- Abstract
Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland-adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
- Subjects
GUINEA pigs as laboratory animals; CYTOMEGALOVIRUS disease treatment; CONGENITAL disorders; CYTOMEGALOVIRUS diseases; INFECTIOUS disease transmission; CYTOMEGALOVIRUSES
- Publication
Viruses (1999-4915), 2014, Vol 6, Issue 2, p448
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v6020448