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- Title
Cerebellar Neurons and Glial Cells Are Transducible by Lentiviral Vectors without Decrease of Cerebellar Functions.
- Authors
Croci, C.; Fasano, S.; Superchi, D.; Perani, L.; Martellosio, A.; Brambilla, R.; Consalez, G.; Bongarzone, E. R.
- Abstract
Due to the profuse connections of the cerebellum to the rest of the central nervous system, cerebellar dysfunction impacts tremendously on movement coordination, maintenance of equilibrium, muscle tone and motor memory. Efficient gene transfer of therapeutic genes to this central nervous system structure would constitute a relevant step ahead the design of treatments to ameliorate cerebellar dysfunction. Lentiviral vectors (LVs) have been used as efficient vehicles to integrate transgenes into dividing and non-dividing cells, such as postmitotic adult neurons, with minimal toxicity and immune response. This study aimed to use LVs carrying green fluorescent protein (GFP) cDNA for transduction of cerebellar cells in vivo without compromising neurological cerebellar functions. Our results indicate that LVs, injected in the lobulus simplex, transduced different cerebellar neurons including stellate, Purkinje cells, granular neurons and glial cells such as astrocytes, oligodendrocytes, and that this gene transfer approach was not accompanied by cerebellar deficits. Copyright © 2006 S. Karger AG, Basel
- Subjects
CEREBELLUM; GREEN fluorescent protein; GENETIC transformation; NEUROGLIA; CENTRAL nervous system; MUSCLE contraction; NEUROSCIENCES
- Publication
Developmental Neuroscience, 2006, Vol 28, Issue 3, p216
- ISSN
0378-5866
- Publication type
Article
- DOI
10.1159/000091919