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- Title
Simultaneous determination of 10 components in traditional Chinese medicine Dachaihu Granule by reversed-phase-high-performance liquid chromatographic-diode array detector.
- Authors
Yingfei Hu; Tulin Lu; Chunqin Mao; Hao Wu; Xing Zhang; JV Wang; Juanjuan Gu
- Abstract
Background: Dachaihu Granule, commonly used for treating cholecystitis, is derived from a famous traditional Chinese formula named Dachaihu Decoction. No analytical method has been reported for simultaneous determination of 10 bioactive compounds for quality control in Dachaihu Granule so far. Objective: To develop a high-performance liquid chromatographic (HPLC) method with diode array detector (DAD) for simultaneous determination of 10 bioactive compounds (paeoniflorin, aloeemodin, rhein, emodin, chrysophanol, physcion, naringin, hesperidin, neohesperidin, and baicalin) in traditional Chinese medicine Dachaihu Granule. Materials and Methods: The samples were separated on a Kromasil C18 (250 × 4.6 mm,i.d. with 5.0 μm particle size)column with multi-wavelength detection method by a gradient elution using acetonitrile (A) and 0.2% acetic acid (B) as the mobile phase. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for paeoniflorin, 254 nm for aloe-emodin, rhein, emodin, chrysophanol, and physcion, 280 nm for naringin, hesperidin, neohesperidin, and baicalin. Results: The developed method provided satisfactory precision and the accuracy of this method was in the range from 94.0% to 103.1%, all of the 10 compounds showed good linearity (r > 0.999) in a detected concentration range. Conclusion: The validated method was successfully applied to the simultaneously of these active components in Dachaihu Granule from different production batches.
- Subjects
CHINESE medicine; HIGH performance liquid chromatography; CHOLECYSTITIS; ACETONITRILE; QUALITY control; THERAPEUTICS
- Publication
Pharmacognosy Magazine, 2013, Vol 9, Issue 33, p33
- ISSN
0973-1296
- Publication type
Article
- DOI
10.4103/0973-1296.108136