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- Title
Semiquinone oscillations as a tool for investigating the ubiquinone binding to photosynthetic reaction centers.
- Authors
Ciriaco, Fulvio; Tangorra, Rocco; Antonucci, Alessandra; Giotta, Livia; Agostiano, Angela; Trotta, Massimo; Milano, Francesco
- Abstract
Semiquinone oscillations induced by light pulses in the presence of exogenous electron donors are a valuable source of information on the kinetics and thermodynamics of ubiquinone chemistry relevant to the Q site of the photosynthetic reaction center (RC). In previous attempts to achieve the quantitative interpretation of data, the ubiquinone concentration was considered constant during the experiment since it was much bigger than that of RC. In this work, we extended existing models to low ubiquinone concentrations revealing several hidden processes taking place during the ubiquinone photoreduction and enabling the evaluation of the ubiquinone binding constant K at the Q site. The proposed approach provides for the first time the evaluation of K without any preliminary treatment of ubiquinone extraction from the binding site, thereby better preserving its native structure.
- Subjects
SEMIQUINONE; UBIQUINONES; PHOTOSYNTHETIC reaction centers; ELECTRON donors; PHOTOREDUCTION; THERMODYNAMICS
- Publication
European Biophysics Journal, 2015, Vol 44, Issue 3, p183
- ISSN
0175-7571
- Publication type
Article
- DOI
10.1007/s00249-015-1013-1