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- Title
Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum.
- Authors
Ritter Antiqueira, Lia Maris Orth; Dequigiovanni, Gabriel; Tambarussi, Evandro Vagner; Rodrigues, Jucelene Fernandes; Veasey, Elizabeth Ann
- Abstract
Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index (FIS) ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.
- Subjects
ORCHIDS; FLOWER varieties; CULTIVARS; MICROSATELLITE repeats; PLANT breeding
- Publication
Crop Breeding & Applied Biotechnology, 2016, Vol 9, Issue 4, p355
- ISSN
1518-7853
- Publication type
Article
- DOI
10.1590/1984-70332016v16n4n52