We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8.
- Authors
Shrivastava, Smriti; Shukla, Pratyoosh; Mukhopadhyay, Kunal
- Abstract
Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry.
- Publication
3 Biotech, 2011, Vol 1, Issue 4, p255
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-011-0032-6