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- Title
Kinetics of the Reaction between a Monoclonal IgM Anti-I and Rabbit Red Cells I. Intact and Hybrid Macroglobulin Molecules and Hybrid Subunits.
- Authors
Solheim, B. G.
- Abstract
Rabbit red cells were found to bind a maximum of 1.8 X 105 IgM anti-I-molecules. A K-value of 5.0 ± 0.8 X 108 I/M was determined for the reaction between the cells and intact anti-1 at 5 °C; the K-value was little affected by changes in temperature. Hybrid 17S and 8S molecules composed of varying amounts of cold agglutinin and inactive IgM were prepared by reduction with 2-mercaptoethanol and reassociation. The results obtained with the reassociated products indicate random reassociation at the half subunit level. It is concluded that a 17S fraction with four or more active half subunits shows binding similar to intact IgM, while 8S fractions with two active half subunits show binding similar to the cysteine-reduced 8S IgM.
- Subjects
ERYTHROCYTES; LABORATORY rabbits; AGGLUTININS; CELLS; IMMUNOGLOBULIN M
- Publication
Scandinavian Journal of Immunology, 1972, Vol 1, Issue 2, p179
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1972.tb00597.x