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- Title
SDF-1 Induces IL-8 Production and Transendothelial Migration of Human Cord Blood-Derived Mast Cells.
- Authors
Lin; Issekutz; Marshall, Jean S.
- Abstract
Background: Mast cell numbers and expression of chemokines are known to increase in the context of angiogenesis and inflammation, but the mechanisms by which this occurs are not understood. Stromal-derived factor-1 (SDF-1) is an important chemokine in angiogenesis and cell migration. The effects of SDF-1 on human mast cells were examined. Methods: Expression of the SDF-1 receptor CXC chemokine receptor 4 (CXCR4) on mast cells was examined by RT-PCR and flow cytometry. The ability of labeled cord blood-derived mast cells to migrate across HUVEC monolayers in response to SDF-1 was determined. The cytokine and chemokine responses of cord blood-derived mast cells to SDF-1 treatment over 24 h were examined by ELISA. Results: Cord blood-derived human mast cells expressed the CXCR4 receptor for SDF-1 and migrated across HUVEC monolayers in response to this chemokine. Treatment of cord blood-derived mast cells with SDF-1 did not induce degranulation or the production of several cytokines but did induce a highly selective IL-8 response. Conclusion: Human mast cells can both migrate across vascular endothelium and produce the pro-angiogenic chemokine IL-8 in response to SDF-1. These responses may be important in angiogenic processes.Copyright © 2001 S. Karger AG, Basel
- Subjects
INTERLEUKIN-8; INTERLEUKINS; CORD blood; MAST cells; CONNECTIVE tissue cells
- Publication
International Archives of Allergy & Immunology, 2001, Vol 124, Issue 1-3, p142
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000053693