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- Title
Cas9 gRNA engineering for genome editing, activation and repression.
- Authors
Kiani, Samira; Chavez, Alejandro; Tuttle, Marcelle; Hall, Richard N; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; Pruitt, Benjamin W; Beal, Jacob; Vora, Suhani; Buchthal, Joanna; Kowal, Emma J K; Ebrahimkhani, Mohammad R; Collins, James J; Weiss, Ron; Church, George
- Abstract
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
- Subjects
TRANSCRIPTION factors; NUCLEASES; GENOME editing; GENETIC engineering; CHEMICAL synthesis
- Publication
Nature Methods, 2015, Vol 12, Issue 11, p1051
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.3580