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- Title
High-throughput single-molecule optofluidic analysis.
- Authors
Kim, Soohong; Streets, Aaron M; Lin, Ron R; Quake, Stephen R; Weiss, Shimon; Majumdar, Devdoot S
- Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
- Subjects
MICROFLUIDICS; BIOMOLECULES; ESCHERICHIA coli; RNA polymerases; CONFORMATIONAL analysis
- Publication
Nature Methods, 2011, Vol 8, Issue 3, p242
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1569