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- Title
Amplification-free digital gene expression profiling from minute cell quantities.
- Authors
Ozsolak, Fatih; Ting, David T.; Wittner, Ben S.; Brannigan, Brian W.; Paul, Suchismita; Bardeesy, Nabeel; Ramaswamy, Sridhar; Milos, Patrice M.; Haber, Daniel A.
- Abstract
Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)+ RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.
- Subjects
STEM cells; GENE amplification; CYTOLOGICAL research; RNA; PANCREATIC duct; CANCER
- Publication
Nature Methods, 2010, Vol 7, Issue 8, p619
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1480