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- Title
Yeast Barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiers.
- Authors
Zhun Yan; Costanzo, Michael; Heisler, Lawrence E.; Paw, Jadine; Kaper, Fiona; Andrews, Brenda J.; Boone, Charles; Giaever, Guri; Nislow, Corey
- Abstract
The ability to perform complex bioassays in parallel enables experiments that are otherwise impossible because of throughput and cost constraints. For example, highly parallel chemical-genetic screens using pooled collections of thousands of defined Saccharomyces cerevisiae gene deletion strains are feasible because each strain is bar-coded with unique DNA sequences. It is, however, time-consuming and expensive to individually bar-code individual strains. To provide a simple and general method of barcoding yeast collections, we built a set of donor strains, called Barcoders, with unique bar codes that can be systematically transferred to any S. cerevisiae collection. We applied this technology by generating a collection of bar-coded 'decreased abundance by mRNA perturbation' (DAmP) loss-of-function strains comprising 87.1% of all essential yeast genes. These experiments validate both the Barcoders and the DAmP strain collection as useful tools for genome-wide chemical-genetic assays.
- Subjects
YEAST; SACCHAROMYCES cerevisiae; SACCHAROMYCES; GENES; GENETICS
- Publication
Nature Methods, 2008, Vol 5, Issue 8, p719
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1231