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- Title
HMGB1/RAGE induces IL-17 expression to exaggerate inflammation in peripheral blood cells of hepatitis B patients.
- Authors
JooYeon Jhun; SeungHoon Lee; HeeYeon Kim; Yang-Mi Her; Jae Kyeong Byun; Eun-Kyung Kim; Soon Kyu Lee; Mi-La Cho; Jong Young Choi; Jhun, JooYeon; Lee, SeungHoon; Kim, HeeYeon; Her, Yang-Mi; Byun, Jae Kyeong; Kim, Eun-Kyung; Lee, Soon Kyu; Cho, Mi-La; Choi, Jong Young
- Abstract
<bold>Background: </bold>Hepatitis B (HB) is an infectious disease with unfavorable consequence for patients and involved in chronic inflammation of liver. The present study aimed to investigate whether High-mobility group protein B (HMGB)1/receptor for advanced glycation end products (RAGE) aggravates inflammation enhancing the expression of interleukin (IL)-17.<bold>Methods: </bold>Mild and severe HB liver tissue and peripheral blood samples were obtained intra-operatively. Histological analysis of the livers was performed by immunohistochemistry. IL-1β and IL-6 of liver tissue were detected by confocal microscopy staining. Relative mRNA expression was measured by real-time PCR and protein levels were measured by enzyme-linked immunosorbent assay.<bold>Results: </bold>HMGB1, RAGE and IL-17 expression is increased in liver of HB patients with acute on chronic liver failure (ACLF) compared to healthy controls. HMGB1 treatment induced inflammatory cytokines including IL-17 in peripheral blood cells of HB patients. IL-17 also induced the expression of RAGE and IL-1β in peripheral blood cells of HB patients with ACLF. On the other hands, the inhibitory factor of p38 and nuclear factor-kappa B reduced the expression of RAGE and IL-1β in peripheral blood cells HB patients with ACLF.<bold>Conclusions: </bold>HMGB1, RAGE and IL-17 expression is increased in liver of severe HB patients. HMGB1 and RAGE interaction may contribute to the inflammation of liver enhancing the expression of IL-17, which can be possibly restored through the decline of the HMGB1/RAGE axis.
- Subjects
PROTEIN metabolism; RNA metabolism; CELL receptors; ENZYME-linked immunosorbent assay; GENES; HEPATITIS B; IMMUNOHISTOCHEMISTRY; INFLAMMATION; INTERLEUKIN-1; INTERLEUKINS; LIVER; LONGITUDINAL method; MICROSCOPY; POLYMERASE chain reaction; TRANSFERASES; CASE-control method
- Publication
Journal of Translational Medicine, 2015, Vol 13, Issue 1, p1
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-015-0663-1