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- Title
Chlamydia Outer Protein (Cop) B from Chlamydia pneumoniae possesses characteristic features of a type III secretion (T3S) translocator protein.
- Authors
Bulir, David C.; Waltho, Daniel A.; Stone, Christopher B.; Liang, Steven; Chiang, Christopher K. W.; Mwawasi, Kenneth A.; Nelson, Jordan C.; Zhang, Steven W.; Mihalco, Samantha P.; Scinocca, Zachariah C.; Mahony, James B.
- Abstract
Background: Chlamydia spp. are believed to use a conserved virulence factor called type III secretion (T3S) to facilitate the delivery of effector proteins from the bacterial pathogen to the host cell. Important early effector proteins of the type III secretion system (T3SS) are a class of proteins called the translocators. The translocator proteins insert into the host cell membrane to form a pore, allowing the injectisome to dock onto the host cell to facilitate translocation of effectors. CopB is a predicted hydrophobic translocator protein within the chlamydial T3SS. Results: In this study, we identified a novel interaction between the hydrophobic translocator, CopB, and the putative filament protein, CdsF. Furthermore, we identified a conserved PxLxxP motif in CopB (amino acid residues 166-171), which is required for interaction with its cognate chaperone, LcrH_1. Using a synthetic peptide derived from the chaperone binding motif of CopB, we were able to block the LcrH_1 interaction with either CopB or CopD; this CopB peptide was capable of inhibiting C. pneumoniae infection of HeLa cells at micromolar concentrations. An antibody raised against the N-terminus of CopB was able to inhibit C. pneumoniae infection of HeLa cells. Conclusion: The inhibition of the LcrH_1:CopB interaction with a cognate peptide and subsequent inhibition of host cell infection provides strong evidence that T3S is an essential virulence factor for chlamydial infection and pathogenesis. Together, these results support that CopB plays the role of a hydrophobic translocator.
- Subjects
CHLAMYDIA; MICROBIAL virulence; ENDOCRINE secretions; BIOLOGICAL transport; TRANSLOCATOR proteins
- Publication
BMC Microbiology, 2015, Vol 15, Issue 1, p1
- ISSN
1471-2180
- Publication type
Article
- DOI
10.1186/s12866-015-0498-1