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- Title
Eye bank versus surgeon prepared Descemet stripping automated endothelial keratoplasty tissues: Influence on adhesion force in a pilot study.
- Authors
Romano, Vito; Parekh, Mohit; Kazaili, Ahmed; Steger, Bernhard; Akhtar, Riaz; Ferrari, Stefano; Kaye, Stephen; Levis, Hannah; Kaye, Stephen B; Levis, Hannah J
- Abstract
<bold>Purpose: </bold>To evaluate and compare the biomechanical properties of the eye bank-prepared and surgeon prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues.<bold>Methods: </bold>In this laboratory study, corneal tissues for research were randomly allocated in the following groups: a) surgeon-cut DSAEK and b) eye bank-prepared (pre-cut and pre-loaded) DSAEK. Endothelial cell loss (ECL), immunostaining for tight junction protein ZO-1, elastic modulus, and adhesion force were investigated.<bold>Results: </bold>ECL was not found to be significantly different between surgeon-cut DSAEK (7.8% ±6.5%), pre-cut DSAEK (8.6% ±2.3%), and pre-loaded DSAEK (11.1% ±4.8%) (P = 0.5910). ZO-1 was expressed equally across all groups. Surgeon-cut DSAEK grafts showed a significantly higher elastic modulus compared to pre-cut and pre-loaded DSAEK groups (P = 0.0047 and P < 0.0001, respectively). Adhesion force was significantly greater in the surgeon-cut DSAEK compared to pre-cut (P < 0.0001) or pre-loaded DSAEK groups (P = 0.0101).<bold>Conclusion: </bold>The laboratory data on the biomechanics of DSAEK grafts suggests that surgeon-cut DSAEK grafts present higher elastic modulus and adhesion force compared to eye bank-prepared DSAEK grafts.
- Subjects
DESCEMET stripping automated endothelial keratoplasty; TISSUE adhesions; CORNEA surgery; PILOT projects; ENDOTHELIUM; TISSUE banks; CORNEA diseases
- Publication
Indian Journal of Ophthalmology, 2022, Vol 70, Issue 2, p523
- ISSN
0301-4738
- Publication type
journal article
- DOI
10.4103/ijo.IJO_3637_20