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- Title
EttA regulates translation by binding the ribosomal E site and restricting ribosome-tRNA dynamics.
- Authors
Chen, Bo; Boël, Grégory; Hashem, Yaser; Ning, Wei; Fei, Jingyi; Wang, Chi; Gonzalez, Ruben L; Hunt, John F; Frank, Joachim
- Abstract
Cells express many ribosome-interacting factors whose functions and molecular mechanisms remain unknown. Here, we elucidate the mechanism of a newly characterized regulatory translation factor, energy-dependent translational throttle A (EttA), which is an Escherichia coli representative of the ATP-binding cassette F (ABC-F) protein family. Using cryo-EM, we demonstrate that the ATP-bound form of EttA binds to the ribosomal tRNA-exit site, where it forms bridging interactions between the ribosomal L1 stalk and the tRNA bound in the peptidyl-tRNA-binding site. Using single-molecule fluorescence resonance energy transfer, we show that the ATP-bound form of EttA restricts ribosome and tRNA dynamics required for protein synthesis. This work represents the first example, to our knowledge, in which the detailed molecular mechanism of any ABC-F family protein has been determined and establishes a framework for elucidating the mechanisms of other regulatory translation factors.
- Subjects
RIBOSOMES; ESCHERICHIA coli DNA; ESCHERICHIA coli chromosomes; CHEMICAL synthesis; ADENOSINE triphosphate; PROTEIN research
- Publication
Nature Structural & Molecular Biology, 2014, Vol 21, Issue 2, p152
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2741