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- Title
Improved 2-methyl-1-propanol production in an engineered Bacillus subtilis by constructing inducible pathways.
- Authors
Li, Shanshan; Jia, Xiaoqiang; Wen, Jianping
- Abstract
High-level constitutive gene expression can result in cellular metabolic imbalance and limit production. To circumvent these problems, a P-controlled auto-inducible 2-ketoisovalerate biosynthetic pathway and a P-controlled IPTG-inducible Ehrlich pathway were constructed in Bacillus subtilis to modulate gene expression. Based on the precise gene expression characteristics of the two inducible pathways, the optimal IPTG induction time point and dose for 2-methyl-1-propanol biosynthesis were determined as 9.5 h and 300 μM, respectively. Under the optimized conditions, strain BSUΔL-03 with inducible pathways produced up to 3.83 ± 0.46 g 2-methyl-1-propanol/l, which was about 60 % higher than BSUL04 with constitutive pathways.
- Subjects
BACILLUS (Bacteria); GENE expression; GENETIC regulation; BIOCHEMISTRY; BIOSYNTHESIS
- Publication
Biotechnology Letters, 2012, Vol 34, Issue 12, p2253
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-012-1041-1