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- Title
Arsenite Oxidation in Ancylobacter dichloromethanicus As3-1b Strain: Detection of Genes Involved in Arsenite Oxidation and CO Fixation.
- Authors
Andreoni, Vincenza; Zanchi, Raffaella; Cavalca, Lucia; Corsini, Anna; Romagnoli, Cristina; Canzi, Enrica
- Abstract
The aim of this study was to characterize a facultative chemolithotrophic arsenite-oxidizing bacterium by evaluating the growth and the rate of arsenite oxidation and to investigate the genetic determinants for arsenic resistance and CO fixation. The strain under study, Ancylobacter dichloromethanicus As3-1b, in a minimal medium containing 3 mM of arsenite as electron donor and 6 mM of CO-bicarbonate as the C source, has a doubling time (t) of 8.1 h. Growth and arsenite oxidation were significantly enhanced by the presence of 0.01 % yeast extract, decreasing the t to 4.3 h. The strain carried arsenite oxidase ( aioA) gene highly similar to those of previously reported arsenite-oxidizing Alpha-proteobacteria . The RuBisCO Type-I ( cbbL) gene was amplified and sequenced too, underscoring the ability of As3-1b to carry out autotrophic As(III) oxidation. The results suggest that A. dichloromethanicus As3-1b can be a good candidate for the oxidation of arsenite in polluted waters or groundwaters.
- Subjects
ARSENITES; ANCYLOBACTER; CARBON dioxide fixation; OXIDATION; GENE amplification
- Publication
Current Microbiology, 2012, Vol 65, Issue 2, p212
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-012-0149-9