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- Title
Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages.
- Authors
Oliveira, Susana; Ordem Trahamane, Evaristo; Monteiro, Juliana; Santos, Gustavo; Crugeira, Pedro; Sampaio, Fernando; Oliveira, Camila; Neto, Manoel; Pinheiro, Antônio; de Oliveira, Susana; da Ordem Trahamane, Evaristo João; Santos, Gustavo Pires; Neto, Manoel Barral; Pinheiro, Antônio
- Abstract
The aim of this study is to evaluate the effects of ApPDT (antiparasitic photodynamic therapy) on the interaction of Leishmania braziliensis with J774 macrophages, used as a photosensitizer, methylene blue associated with red laser. The tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and ApPDT. The photosensitizer used was the methylene blue at concentration of 12.5 μg/mL. The parameters of the laser were λ = 660 nm, 40 mW, and 8.4 J/cm2. Samples are analyzed by optical microscopy through the identification and counting of infected and uninfected macrophages, parasite load, infectivity, and infection index. Statistical analysis used ANOVA test with Tukey post-test, being considered statistically significant p < 0.05. The analysis of the interaction tests shows that the infection rate in the ApPDT group in relation to the control group presents a statistically significant reduction (p < 0.0001) of 71% at both 24 and 48 h (p < 0.0001) of 62%. ApPDT reduces the number of macrophages infected by Leishmania braziliensis, as well as the number of intracellular parasites, being a possible alternative therapy in the treatment of cutaneous leishmaniosis.
- Subjects
MACROPHAGES; PHOTODYNAMIC therapy; PHOTOSENSITIZERS; ANALYSIS of variance; LASER therapy; ANIMAL experimentation; ANTIPARASITIC agents; LASERS; LEISHMANIA; LEISHMANIASIS; METHYLENE blue; MICE; PARASITES; PHOTOCHEMOTHERAPY; PHARMACODYNAMICS; THERAPEUTICS
- Publication
Lasers in Medical Science, 2017, Vol 32, Issue 9, p1959
- ISSN
0268-8921
- Publication type
journal article
- DOI
10.1007/s10103-017-2292-9