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- Title
Inappropriate annotation of a key defence marker inArabidopsis: will the realPR-1please stand up?
- Authors
Laird, Janet; Armengaud, Patrick; Giuntini, Pietro; Laval, Valérie; Milner, Joel
- Abstract
PR-1has been extensively used as a marker for salicylic acid (SA)-mediated defence and systemic and local acquired resistance. TheArabidopsisGenome Project annotates At2g19990 asPR-1. This gene is also identified asPR-1in two “full genome”Arabidopsismicroarrays, and TAIR cites approximately 60 articles to describe its patterns of expression. However, most of these citations are incorrect; the probes used were not At2g19990, but a homologous gene At2g14610, which is annotated as “PR-1-like”. Because of the potential for confusion, we analyzed the expression of both genes inArabidopsis thaliana(L.) Heynh. At2g14610 (PR-1-like) showed the archetypal patterns of SA-responsive expression: mRNA levels increased following SA-treatment, inoculation with an avirulent (but not a virulent) strain ofPseudomonas syringae, and in wild-type (but notNahG)Arabidopsisinfected with cauliflower mosaic virus (CaMV). Incpr5mutants it was expressed constitutively. In contrast, expression of At2g19990 (annotated asPR-1) was detectable in neither SA-treated Col-0 nor incpr5. Infection by virulent and avirulent isolates ofP. syringaeup-regulated expression, but to a similar level, and infection by CaMV induced a modest increase in expression in both the wild type andNahG. At2g19990, although pathogen responsive, does not show the SA-dependent patterns of expression expected from a member of thePR-1regulon, and its annotation as “PR-1” is inappropriate. The annotations should identify At2g14610 as the authenticPR-1.
- Subjects
ARABIDOPSIS; DISEASE resistance of plants; PHYTOPATHOGENIC microorganisms; PLANT disease etiology; SALICYLIC acid; PLANT genetics
- Publication
Planta: An International Journal of Plant Biology, 2004, Vol 219, Issue 6, p1089
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-004-1355-x