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- Title
Effect of Hyperthermia on Intracellular pH in Human U-87 MG Glioblastoma Cells.
- Authors
Lui, C.P.; Fung, K.P.; Kong, S.K.; Choy, Y.M.; Lee, C.Y.
- Abstract
The effect of hyperthermia at 43° C on intracellular pH (pHi) in human U-87 MG glioblastoma cells was studied by using the fluorescent probe 2',7'-bis(car-boxyethyl)-5(6)-carboxyfluorescein-pentaacetoxymethyl ester. The presence of Na+/H+ antiporter activity in the cells were demonstrated by the Na+-dependent increase in intracellular pH (pHi) after cellular acidification in the absence of HCO3-. Hyperthermia at 43 °C caused significant decrease in pHi. The acidification was readily reversible by cooling the cells back down to 37 °C. The pHi change was inhibited by the addition of 1 mM amiloride in the incubation medium. Amiloride and hyperthermia exhibited a synergistic effect in suppressing thymidine incorporation into the cells. Copyright © 1995 S. Karger AG, Basel
- Publication
Oncology, 1995, Vol 52, Issue 6, p492
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000227517