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- Title
Human Liver Cytochrome P-450 Related to a Rat Acetone-Inducible, Nitrosamine-Metabolizing Cytochrome P-450: Identification and Isolation.
- Authors
Robinson, Richard C.; Shorr, Robert G.L.; Varrichio, Angela; Park, Sang S.; Gelboin, Harry V.; Miller, Haruko; Friedman, Fred K.
- Abstract
A monoclonal antibody (MAb) to a rat acetone-inducible and nitrosamine-metabolizing form of microsomal cytochrome P-450, P-450ac, detected a related P-450 in human liver microsomes by both immunoblot and competitive radioimmunoassay. This MAb was also used to immunopurify microsomal cytochromes P-450 from both human liver and acetone-treated rats; these were electrophoretically homogeneous with apparent molecular weights of 56,200 and 53,000 daltons, respectively. The structures of the cytochromes P-450 were compared by peptide mapping and amino-terminal sequence analyses. They differed in their peptide maps but displayed amino-terminal sequence similarity in their first 19 residues. This report thus demonstrates the utility of MAbs to rat cytochromes P-450 for detection, identification and structural characterization of human P-450s. Copyright © 1989 S. Karger AG, Basel
- Publication
Pharmacology, 1989, Vol 39, Issue 3, p137
- ISSN
0031-7012
- Publication type
Article
- DOI
10.1159/000138590