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- Title
Pregnancies following blastocyst stage transfer in PGD cycles at risk for beta-thalassaemic haemoglobinopathies.
- Authors
Palmer, G. A.; Traeger-Synodinos, J.; Davies, S.; Tzetis, M.; Vrettou, C.; Mastrominas, M.; Kanavakis, E.
- Abstract
<bold>Background: </bold>Preimplantation genetic diagnosis (PGD) usually involves blastomere biopsy 3 days post-insemination (p.i.), followed by genetic analysis and transfer of unaffected embryos later on day 3 or 4. We evaluate a strategy involving embryo biopsy on day 3 p.i., genetic analysis on day 4 and, following culture in blastocyst sequential media, transfer of unaffected embryos on day 5 p.i.<bold>Methods: </bold>PGD cycles were initiated in 15 couples at risk of transmitting beta-thalassaemia major. Oocyte retrieval and ICSI were performed according to standard protocols. Embryo culture used blastocyst sequential media. Embryos were biopsied on day 3 p.i. using acid Tyrode's for zona drilling, and the single blastomeres were genotyped by a protocol involving nested polymerase chain reaction and denaturing gradient gel electrophoresis analysis.<bold>Results: </bold>Forty of 109 (37%) embryos biopsied on day 3 p.i. developed to blastocysts by day 5 p.i., with at least one blastocyst available for transfer in 12 cycles (80%). Genotype analysis characterized 51/109 (47%) embryos unaffected for beta-thalassaemia major, of which 28 were blastocysts. Transfer of 37 day 5 p.i. embryos (blastocysts and non blastocysts) initiated eight clinical pregnancies. Implantation rate per embryo transferred was 12/37 (32%).<bold>Conclusions: </bold>Embryo biopsy on day 3, followed by delayed transfer until day 5 p.i. offers a novel and effective strategy to overcome the time limit encountered when performing PGD, without compromising embryo implantation.
- Subjects
BIOPSY; BLASTOCYST; CULTURE media (Biology); DISEASE susceptibility; EMBRYO transfer; FERTILIZATION in vitro; RESEARCH methodology; EVALUATION of medical care; POLYMERASE chain reaction; PREGNANCY; PREIMPLANTATION genetic diagnosis; TIME; TISSUE culture; GENETIC carriers; BETA-Thalassemia; GENOTYPES
- Publication
Human Reproduction, 2002, Vol 17, Issue 1, p25
- ISSN
0268-1161
- Publication type
journal article
- DOI
10.1093/humrep/17.1.25