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- Title
The Comparison of Apoptosis Anti-apoptosis and Autophagy Markers in Epicardial Tissue in Patients with Coronary Artery Disease and in Patients have Valvular Disease, HOCM, Ascending Aortic Aneurysm without Coronary Artery Disease.
- Authors
Karataş, Mesut; Parsova, Kemal Emrecan; Keleş, Nurşen; Özer, Tanıl; Alıcı, Gökhan
- Abstract
Background: Apoptosis is a highly programed mechanism that occurs as a part of cell life. Anti-apoptotic and pro-apoptotic proteins regulate this process. Autophagy is a catabolic process that involves the degradation and recycling of aged cytoplasmic components. In this study, we evaluated the expression rates of bax (apoptosis), Bcl-2 (anti-apoptosis), and beclin (autophagy) proteins in epitadial tissue in cardiovascular diseases. Materials and Methods: Patients with coronary artery by-pass graft (CABG) due to coronary artery disease constituted the patient group. Patients who underwent mitral valve repair (MVR) for severe mitral regurgitation, or who underwent aortic valve replacement (AVR) for severe aortic regurgitation, or who underwent myectomy for hypertrophic obstructive cardiomyopathy (HOCM) or Benthall procedure for ascending aortic aneurysm were included in the study as a control group. Biopsy samples were taken from the epicardial tissues during surgery. Sections taken from the samples were stained with hematoxylin and eosin and examined histopathologically under a light microscope to evaluate inflammation intensity. Samples found suitable were stained immunohistochemically with bronchioloalveolar carcinoma, Bcl-, and Beclin antibodies. Results: Inflammation in patients with CABG was considerably higher than that in the control group. In patients with CABG with inflammation, Bax expression increased in parallel with inflammation. Bcl-2 expression was significantly increased in patients with MVR, AVR, or myectomy. The highest rate of Beclin expression was observed in HOCM patients. Similar results were obtained in the patient who underwent the Benthall procedure as in the HOCM patient. Beclin expression was also increased in patients who underwent AVR or MVR and showed high Bcl-2 expression. Conclusion: All of the participants had Bax, Bcl-2, and Beclin expression, albeit at different levels. Inflammation level is determinative for Bax and Beclin expression. In patients with inflammatory CABG, Bax expression increases in parallel with inflammation. In patients with MVR or AVR or myectomy without ischemic heart disease, Bcl-2 expression increased much more than in patients with CABG.
- Subjects
CORONARY artery disease; APOPTOSIS; AUTOPHAGY; AORTIC valve transplantation; IMMUNOHISTOCHEMISTRY
- Publication
Hamidiye Medical Journal, 2023, Vol 4, Issue 3, p168
- ISSN
2718-0956
- Publication type
Article
- DOI
10.4274/hamidiyemedj.galenos.2023.21033