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- Title
IDENTIFICATION OF VAM SPORES (GLOMUS AGGREGATUM AND GLOMUS MOSSEAE) BASED ON CTAB METHOD IN THE RHIZOSPHERE SOIL OF QUINOA (CHENOPODIUM QUINOA WILLD.).
- Authors
Sandepogu, Prashanthi
- Abstract
The bio-fertilizer properties of plant growth promoting bacteria are frequently ascribed to their ability to increase the bioavailability. Rhizosphere microflora like VAM shown incensement in the growth and yield of some agricultural crops. It is the necessary and primary step to identify the presence of VAM in the rhizosphere soil of the higher plants. So, the standardization of DNA isolation is basic requirement for any further research to be carried out. We report here modified CTAB technique for isolation of genomic DNA from two cultivars of Quinoa (INIA-431, INIA - 427) (Chenopodium quinoa Willd.) We got very good yield of DNA samples from the rhizosphere soil of Quinoa. Genomic DNA isolated by modified CTAB method was pure; the highest level of purity was obtained from two cultivars of Quinoa. The two cultivars of Quinoa (INIA - 431, INIA - 427) gave good yield of DNA from the established modified CTAB protocol. Identification of spores (Glomus aggregatum and Glomus mosseae) based on CTAB method in the Rhizosphere soil of Quinoa (Chenopodium quinoa Willd.)
- Subjects
GLOMUS (Fungi); RHIZOSPHERE; QUINOA; PLANT growth; BIOFERTILIZERS
- Publication
Plant Archives (09725210), 2021, Vol 21, Issue 2, p645
- ISSN
0972-5210
- Publication type
Article
- DOI
10.51470/PLANTARCHIVES.2021.v21.no2.098