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- Title
采用MiSeq测序技术分析3种飞虱中肠内容物的菌群结构.
- Authors
张珏锋; 俞叶飞; 李芳; 钟海英; 陈建明
- Abstract
In this study, we successfully demonstrated that MiSeq was a powerful approach for investigating the bacterial communities in midgut of brown planthopper ( BPH) Nilaparvata lugens Stal, white-backed planthopper ( WBPH) Sogatella furcifera Horvath and small brown planthopper ( SBPH) L=delphax striatellus (Fallen). For all three planthoppers, 3. 44 x 105 effective sequences were selected and utilized for the bacterial diversity and abundance analysis. In total, 76, 103, and 61 operational taxonomic units were obtained at 3 % distance cutoff, 49, 68 and 42 at 6% distance cutoff in the BPH, SBPH and WBPH, respectively. Nearly 90% of the sequences in the SBPH were assigned into F-proteobacteria-unclassified. In the other two samples, Arsenophonus was the dominant genus in BPH, accounting for about about 70% sequences, while in WBPH, Halomonas, Candidatus_Cardinium and Arsenophonus were 3 dominant bacterias, accounting for about 35 %, 20% and 15%, respectively. Though they were evolved from the common ancestor, the microbial community diversity, richness, dominant bacteria function of endosymbiont in three planthoppers were different.
- Publication
Acta Agriculturae Zhejiangensis, 2020, Vol 32, Issue 12, p2192
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2020.12.10