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- Title
利用 EMS 诱变创制抗除草剂粳稻新种质.
- Authors
王付华; 李自超; 王 亚; 付 景; 杨文博; 尹海庆; 王生轩; 王越涛; 白 涛; 张 珍
- Abstract
In order to create new herbicide-resistant rice germplasms,the dry seeds of Zhengdao 19 were soaked in ethylmethane sulfonate ( EMS) solution. At three-leaf stage, M2 seedlings were sprayed with imazapic(112. 5 g / ha) and nicosulfuron( 60 g / ha) . The herbicide-resistant mutants were screened after 14 days. The ALS ( acetolactate synthase ) gene sequences of mutants were analyzed to find out the mutation sites. The results showed that the herbicide-resistant plants grew normally after spraying imazapic,the leaf kept green,while the leaves of sensitive lines yellowed,and their growth were inhibited; after spraying nicosulfuron,the growth of herbicide-resistant plants were inhibited in the early stage,but could recover slowly. A total of 63 000 M2 lines were screened with imazapic, and 6 imazapic-resistant mutants (HF1—HF6) were obtained,including two types of ALS mutations,Ser-627-Asn and Gly-628- Glu. A total of 30 000 M2 lines were screened with nicosulfuron, and 2 herbicide-resistant mutants (HF7—HF8) were obtained, including two types of ALS mutations, Pro-171-Ser and Ala-179-Val. To obtain a specific ALS nucleotide mutation,an average of 23 250 M2 lines were needed to screen. The fresh weight reduction rates of imazapic-resistant mutants HF1 ( Gly-628-Glu ) and HF2 ( Ser-627-Asn ) were 13. 09% and 20. 03% respectively after spraying 1 time the conventional herbicide dose( 112. 5 g / ha), which were significantly less than that of Zhengdao 19 (P<0. 05) ; the mutants could still grow normally after spraying 8 times the conventional herbicide dose in the field,showing strong resistance to imazapic herbicides. The fresh weight reduction rates of mutants HF1 and HF2 were 24. 81% and 13. 13% after spraying 30 g / ha nicosulfuron, and were 34. 13% and 28. 01% after spraying 60 g / ha nicosulfuron, respectively,which were significantly less than those of Zhengdao 19 ( P < 0. 05 ), showing certain nicosulfuron resistance. In summary, 8 herbicide-resistant mutant lines were obtained, 2 lines were resistant to nicosulfuron, 6 lines were resistant to imazapic, and two of the imazapic-resistant mutants exhibited nicotinsulfuron resistance. The screened herbicide-resistant mutants could be used to breed nonGMO herbicide-resistant varieties,and also be used as a basic germplasm for the multi-site mutation of ALS,so as to further strengthen the herbicide resistance of mutants.
- Subjects
UPLAND rice; ACETOLACTATE synthase; HERBICIDES; PLANT growth; RICE drying; HERBICIDE resistance; EFFECT of herbicides on plants; HERBICIDE-resistant crops
- Publication
Journal of Henan Agricultural Sciences, 2021, Vol 50, Issue 4, p8
- ISSN
1004-3268
- Publication type
Article
- DOI
10.15933/j.cnki.1004-3268.2021.04.002