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- Title
Amplified fragment length polymorphism amongRhynchosporium secalisisolates collected from a single barley field in Syria.
- Authors
Kiros-Meles, A.; Udupa, S.; Abang, M. M.; Abu-Blan, H.; Baum, M.; Ceccarelli, S.; Yahyaoui, A. H.
- Abstract
AFLP markers were used to measure the amount and distribution of genetic variation amongRhynchosporium secalisisolates on a microgeographical scale in Syria. Forty isolates hierarchically sampled from a single barley field were assayed for AFLP variation using primer combinations not previously tested in populations of the pathogen from Syria. In contrast to a previous study, which showed high clonality within field populations ofR. secalisin Syria, the present study revealed a much higher level of genetic diversity, stressing the important roles that sampling strategies and the choice of primers/primer combinations play in the evaluation of genetic variation inR. secalispopulations at a microgeographical scale. A high level of genetic variation was found to occur on a fine scale throughout the pathogen population examined, with 40 different haplotypes being identified among the 40 isolates sampled. Data were consistent with the hypothesis that the primary inoculum originated from a genetically diverse founding population, which may have consisted of ascospores of an as yet undescribed teleomorph and/or asexual spores of a highly mutable local population.
- Subjects
SYRIA; GENETIC polymorphisms; RHYNCHOSPORIUM secalis; PATHOGENIC microorganisms; GENETICS
- Publication
Annals of Applied Biology, 2005, Vol 146, Issue 3, p389
- ISSN
0003-4746
- Publication type
Article
- DOI
10.1111/j.1744-7348.2005.040139.x