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- Title
Biotechnological parameters determination for cultivation of lactic acid bacteria from goat milk.
- Authors
Bogdan, Nina
- Abstract
Introduction. The purpose of research - to establish optimal cultivation conditions to determine of the efficacy of development lactic acid bacteria isolated from goat milk. Materials and methods. Strains cultivation were performed in sterilized goat milk medium at the 30±2 °C. Parameters determination have been established for the periodic cultivation in bioreactor Sartorius Biostat® A plus. Count enumeration of lactic acid bacteria cells was performed using the spread count method. Results and discussion. The slow pH decrease was observed at all selected strains during cultivation time. Lactic acid has been accumulated proportional what shows the intense development of the lactic acid bacteria up to 14 cultivation hours. The active development is characterized through moderate acidity of fermented milk. Data of strains development dynamics and the biomass accumulation in the medium goat milk-based proved the obtaining to maximum count of lactic acid bacteria cells lg CFU/g. At the same time the regression analysis of the pH value dinamics and viable cells count were performed for exactly describes results of the experiments. The strains CNMN-LB-73, CNMN-LB-74, CNMN-LB-77 and CNMN-LB-78 has the accurate regression line at pH dinamic and strains CNMN-LB-73, CNMN-LB-74, CNMN-LB-75, CNMN-LB-76 and CNMN-LB-78 at the dynamic of cells development. Strains development stages have observed since 10 hours of cultivation. The decline phase started after 12 hours for CNMN-LB-76, CNMN-LB-77, CNMN-LB-79 strains and after 14 hours for CNMN-LB-73, CNMN-LB-74, CNMN-LB-75, CNMN-LB-78, indicating that they are more resistant to acid conditions that isolated strains reported by other authors, cell multiplication was observed until the pH 4.7. Conclusions. In conditions of periodic cultivation biotechnological parameters for cultivation of lactic acid bacteria from goat milk were determinated. Obtained data demonstrated important biotechnological properties of isolated strains.
- Subjects
LACTIC acid bacteria; GOAT milk
- Publication
Ukrainian Food Journal, 2018, Vol 7, Issue 4, p605
- ISSN
2304-974X
- Publication type
Article
- DOI
10.24263/2304-974X-2018-7-4-6