We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Azolla domestication towards a biobased economy?
- Authors
Brouwer, Paul; Bräutigam, Andrea; Külahoglu, Canan; Tazelaar, Anne O. E.; Kurz, Samantha; Nierop, Klaas G. J.; Werf, Adrie; Weber, Andreas P. M.; Schluepmann, Henriette
- Abstract
Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome., Methods for dissemination, cross-fertilization and cryopreservation of the symbiosis Azolla filiculoides-Nostoc azollae are tested based on the fern spores. To study molecular processes in Azolla including spore induction , a database of 37 649 unigenes from RNAseq of microsporocarps, megasporocarps and sporophytes was assembled, then validated., Spores obtained year-round germinated in vitro within 26 d. In vitro fertilization rates reached 25%. Cryopreservation permitted storage for at least 7 months. The unigene database entirely covered central metabolism and to a large degree covered cellular processes and regulatory networks. Analysis of genes engaged in transition to sexual reproduction revealed a FLOWERING LOCUS T-like protein in ferns with special features induced in sporulating Azolla fronds., Although domestication of a fern-cyanobacteria symbiosis may seem a daunting task, we conclude that the time is ripe and that results generated will serve to more widely access biochemicals in fern biomass for a biobased economy.
- Subjects
AZOLLA; DOMESTICATION of plants; NITROGEN fertilizers; CYANOBACTERIA; FERNS; AZOLLA filiculoides; PLANT reproduction
- Publication
New Phytologist, 2014, Vol 202, Issue 3, p1069
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.12708