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- Title
Ozonation of Whole Blood Results in an Increased Release of Microparticles from Blood Cells.
- Authors
Boczkowska-Radziwon, Barbara; Olbromski, Piotr Józef; Rogowska, Anna; Bujno, Magdalena; Myśliwiec, Marta; Żebrowska, Agnieszka; Średziński, Dariusz; Polityńska, Barbara; Wojtukiewicz, Marek Z.; Radziwon, Piotr
- Abstract
Autohemotherapy with ozonated blood is used in the treatment of a broad spectrum of clinical disorders. Ozone demonstrates strong oxidizing properties and causes damage to cell membranes. The impact of whole-blood ozonation on the release of microparticles from blood and endothelial cells and the concentration of selected markers in the hemostatic system (APTT, PT, D-dimer, fibrinogen) were investigated. Venous blood, obtained from 19 healthy men, was split into four equal parts and treated with air, 15 µg/mL ozone, or 30 µg/mL ozone, or left untreated. The number and types of microparticles released were determined using flow cytometry on the basis of surface antigen expression: erythrocyte-derived microparticles (CD235+), platelet-derived microparticles (CD42+), leukocyte-derived microparticles (CD45+), and endothelial-derived microparticles (CD144+). The study is the first to demonstrate that ozone induces a statistically significant increase in the number of microparticles derived from blood and endothelial cells. Although statistically significant, the changes in some coagulation factors were somewhat mild and did not exceed normal values.
- Subjects
BLOOD coagulation factors; OZONIZATION; CELL surface antigens; ENDOTHELIAL cells; CELL membranes; ERYTHROCYTES; BLOOD cells
- Publication
Biomolecules (2218-273X), 2022, Vol 12, Issue 2, p164
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom12020164