We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Campylobacter jejuni Modulates Reactive Oxygen Species Production and NADPH Oxidase 1 Expression in Human Intestinal Epithelial Cells.
- Authors
Hong, Geunhye; Davies, Cadi; Omole, Zahra; Liaw, Janie; Grabowska, Anna D.; Canonico, Barbara; Corcionivoschi, Nicolae; Wren, Brendan W.; Dorrell, Nick; Elmi, Abdi; Gundogdu, Ozan
- Abstract
Campylobacter jejuni is the major bacterial cause of foodborne gastroenteritis worldwide. Mechanistically, how this pathogen interacts with intrinsic defence machinery of human intestinal epithelial cells (IECs) remains elusive. To address this, we investigated how C. jejuni counteracts the intracellular and extracellular reactive oxygen species (ROS) in IECs. Our work shows that C. jejuni differentially regulates intracellular and extracellular ROS production in human T84 and Caco-2 cells. C. jejuni downregulates the transcription and translation of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (NOX1), a key ROS-generating enzyme in IECs and antioxidant defence genes CAT and SOD1. Furthermore, inhibition of NOX1 by diphenylene iodonium (DPI) and siRNA reduced C. jejuni ability to interact, invade, and intracellularly survive within T84 and Caco-2 cells. Collectively, these findings provide mechanistic insight into how C. jejuni modulates the IEC defence machinery.
- Subjects
CAMPYLOBACTER jejuni; REACTIVE oxygen species; EPITHELIAL cells; NICOTINAMIDE adenine dinucleotide phosphate; INTESTINES
- Publication
Cellular Microbiology, 2023, p1
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1155/2023/3286330