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- Title
Recombinant Sendai virus provides a highly efficient gene transfer into human cord blood-derived hematopoietic stem cells.
- Authors
Jin, C H; Kusuhara, K; Yonemitsu, Y; Nomura, A; Okano, S; Takeshita, H; Hasegawa, M; Sueishi, K; Hara, T
- Abstract
Hematopoietic stem cells (HSCs) are a promising target for gene therapy, however, the low efficiencies of gene transfer using currently available vectors face practical limitations. We have recently developed a novel and efficient gene transfer agent, namely recombinant Sendai virus (SeV), and we have here characterized SeV-mediated gene transfer to human cord blood (CB) HSCs and primitive progenitor cells (PPC) using the jelly fish green fluorescent protein (GFP) gene. Even at a relatively low titer (10 multiplicity of infections), SeV achieved highly efficient GFP expression in CB CD34+ cells (85.5±5.8%), as well as more immature CB progenitor cells, CD34+AC133+ (88.2±3.7%) and CD34+CD38- (84.6±5.7%) cells, without cytokines prestimulation, that was a clear contrast to the features of gene transfer using retroviruses. SeV-mediated gene transfer was not seriously affected by the cell cycle status. In vitro cell differentiation studies revealed that gene transfer occurred in progenitor cells of all lineages (GM-CFU, 73.0±11.1%; BFU-E, 24.7±4.0%; Mix-CFU, 59±4.0%; and total, 50.0±7.0%). These findings show that SeV could prove to be a promising vector for efficient gene transfer to CB HSCs, while preserving their ability to reconstitute the entire hematopoietic series.Gene Therapy (2003) 10, 272–277. doi:10.1038/sj.gt.3301877
- Subjects
SENDAI virus; GENETIC transformation; CORD blood; HEMATOPOIETIC stem cells
- Publication
Gene Therapy, 2003, Vol 10, Issue 3, p272
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3301877