We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
IL-7 production in murine lymphatic endothelial cells and induction in the setting of peripheral lymphopenia.
- Authors
Miller, Corey N.; Hartigan-O’Connor, Dennis J.; Lee, Myeong Sup; Laidlaw, Grace; Cornelissen, Ivo P.; Matloubian, Mehrdad; Coughlin, Shaun R.; McDonald, Donald M.; McCune, Joseph M.
- Abstract
IL-7 is a required factor for T-cell homeostasis. Because of low expression levels and poor reagent availability, the cellular sources of IL-7 have proven challenging to characterize. In this study, we describe a reporter mouse in which enhanced GFP is expressed from the endogenous Il7 locus. We show that IL-7 is produced by lymphatic endothelial cells (LECs) distributed throughout the systemic lymphatic vasculature as well as by fibroblastic reticular cells, and that phosphorylation of STAT5 in lymphocytes is higher in lymphatics than in blood. Furthermore, in nodes depleted of lymphocytes, Il7 transcription is increased in stromal but not in myeloid subsets. These data support recent findings that lymphocyte homeostasis is influenced by access to secondary lymphoid organs and point to LECs as an important in vivo source of IL-7, bathing trafficking immune cells under both resting and lymphopenic conditions.
- Subjects
INTERLEUKIN-7; LYMPHOPENIA; LABORATORY mice; T cells; ENDOTHELIAL cells; GREEN fluorescent protein; LYMPHOCYTES
- Publication
International Immunology, 2013, Vol 25, Issue 8, p471
- ISSN
0953-8178
- Publication type
Article
- DOI
10.1093/intimm/dxt012