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- Title
Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling.
- Authors
Meier, Stefanie S. M.; Multamäki, Elina; Ranzani, Américo T.; Takala, Heikki; Möglich, Andreas
- Abstract
Bacteria must constantly probe their environment for rapid adaptation, a crucial need most frequently served by two-component systems (TCS). As one component, sensor histidine kinases (SHK) control the phosphorylation of the second component, the response regulator (RR). Downstream responses hinge on RR phosphorylation and can be highly stringent, acute, and sensitive because SHKs commonly exert both kinase and phosphatase activity. With a bacteriophytochrome TCS as a paradigm, we here interrogate how this catalytic duality underlies signal responses. Derivative systems exhibit tenfold higher red-light sensitivity, owing to an altered kinase-phosphatase balance. Modifications of the linker intervening the SHK sensor and catalytic entities likewise tilt this balance and provide TCSs with inverted output that increases under red light. These TCSs expand synthetic biology and showcase how deliberate perturbations of the kinase-phosphatase duality unlock altered signal-response regimes. Arguably, these aspects equally pertain to the engineering and the natural evolution of TCSs. Histidine kinases form part of bacterial two-component signaling systems (TCS). By modulating the balance between antagonistic kinase and phosphatase activities, the authors reprogram output and sensitivity of light-responsive TCSs, thus generating tools for gene-expression control by red light.
- Subjects
HISTIDINE kinases; HISTIDINE; SYNTHETIC biology; SCULPTURE; PHOSPHORYLATION; ENGINEERING
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-49251-8