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- Title
Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.
- Authors
Steichen, Seth; Deshpande, Arnav; Mosey, Megan; Loob, Jessica; Douchi, Damien; Knoshaug, Eric P.; Brown, Stuart; Nielsen, Robert; Weissman, Joseph; Carrillo, L. Ruby; Laurens, Lieve M. L.
- Abstract
Carbon capture and biochemical storage are some of the primary drivers of photosynthetic yield and productivity. To elucidate the mechanisms governing carbon allocation, we designed a photosynthetic light response test system for genetic and metabolic carbon assimilation tracking, using microalgae as simplified plant models. The systems biology mapping of high light-responsive photophysiology and carbon utilization dynamics between two variants of the same Picochlorum celeri species, TG1 and TG2 elucidated metabolic bottlenecks and transport rates of intermediates using instationary 13C-fluxomics. Simultaneous global gene expression dynamics showed 73% of the annotated genes responding within one hour, elucidating a singular, diel-responsive transcription factor, closely related to the CCA1/LHY clock genes in plants, with significantly altered expression in TG2. Transgenic P. celeri TG1 cells expressing the TG2 CCA1/LHY gene, showed 15% increase in growth rates and 25% increase in storage carbohydrate content, supporting a coordinating regulatory function for a single transcription factor. Researchers identify unique transcriptional regulation that controls photosynthetic response, growth and biochemical carbon storage in high light for two variants of the same algae species, offering a glimpse into diel control of plant and crop yields.
- Subjects
CIRCADIAN rhythms; TRANSCRIPTION factors; CARBON sequestration; CLOCK genes; PLANT genes; GENE expression; MOLECULAR clock
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-49090-7